Vegetation history and paleoclimate at Lake Dojran ( FYROM / Greece ) during the Late Glacial and Holocene

A new high-resolution pollen and NPP (non-pollen palynomorph) analysis has been performed on the sediments of Lake Dojran, a transboundary lake located at the border between Greece and the former Yugoslav Republic of Macedonia (FYROM). The sequence covers the last 12 500 years and provides information on the vegetational dynamics of the Late Glacial and Holocene for the southern Balkans. Robust age model, sedimentological diatom, and biomarker analyses published previously have been the base for a multi-perspective interpretation of the new palynological data. Pollen analysis revealed that the Late Glacial is characterized by steppic taxa with prevailing Amaranthaceae, Artemisia and Poaceae. The arboreal vegetation starts to rise after 11 500 yr BP, taking a couple of millennia to be definitively attested. Holocene vegetation is characterized by the dominance of mesophilous plants. The Quercus robur type and Pinus are the most abundant taxa, followed by the Quercus cerris type, the Quercus ilex type and Ostrya-Carpinus orientalis. The first attestation of human presence can be presumed at 5000 yr BP from the contemporary presence of cereals, Juglans and Rumex. A drop in both pollen concentration and influx together with a δ 18 O carb shift indicates increasing aridity and precedes clear and continuous human signs since 4000 yr BP. Also, a correlation between Pediastrum boryanum and fecal stanol suggests that the increase in nutrients in the water is related to human presence and pasture. An undoubted expansion of human-related plants occurs since 2600 yr BP when cereals, arboreal cultivated and other synanthropic non-cultivated taxa are found. A strong reduction in arboreal vegetation occurred at 2000 yr BP, when the Roman Empire impacted a landscape undergoing climate dryness in the whole Mediterranean area. In recent centuries the human impact still remains high but spots of natural vegetation are preserved. The Lake Dojran multi-proxy analysis including pollen data provides clear evidence of the importance of this approach in paleoenvironmental reconstruction. Cross-interpretation of several proxies allows us to comprehend past vegetation dynamics and human impact in the southern Balkans. Disciplines Medicine and Health Sciences | Social and Behavioral Sciences Publication Details Masi, A., Francke, A., Pepe, C., Thienemann, M., Wagner, B. & Sadori, L. (2018). Vegetation history and paleoclimate at Lake Dojran (FYROM/Greece) during the Late Glacial and Holocene. Climate of the Past, 14 (3), 351-367. Authors Alessia Masi, Alexander Francke, Caterina Pepe, Matthias Thienemann, Bernd Wagner, and Laura Sadori This journal article is available at Research Online: http://ro.uow.edu.au/smhpapers/5328 Clim. Past, 14, 351–367, 2018 https://doi.org/10.5194/cp-14-351-2018 © Author(s) 2018. This work is distributed under the Creative Commons Attribution 4.0 License. Vegetation history and paleoclimate at Lake Dojran (FYROM/Greece) during the Late Glacial and Holocene Alessia Masi1, Alexander Francke2,3, Caterina Pepe1, Matthias Thienemann2, Bernd Wagner2, and Laura Sadori1 1Department of Environmental Biology, Sapienza University, Rome, Italy 2Institute of Geology and Mineralogy, University of Cologne, Cologne, Germany 3Wollongong Isotope Geochronology Laboratory, School of Earth and Environmental Sciences, University of Wollongong, Wollongong, Australia Correspondence: Alessia Masi (alessia.masi@uniroma1.it) Received: 10 September 2017 – Discussion started: 29 September 2017 Revised: 8 February 2018 – Accepted: 12 February 2018 – Published: 13 March 2018 Abstract. A new high-resolution pollen and NPP (nonpollen palynomorph) analysis has been performed on the sediments of Lake Dojran, a transboundary lake located at the border between Greece and the former Yugoslav Republic of Macedonia (FYROM). The sequence covers the last 12 500 years and provides information on the vegetational dynamics of the Late Glacial and Holocene for the southern Balkans. Robust age model, sedimentological diatom, and biomarker analyses published previously have been the base for a multi-perspective interpretation of the new palynological data. Pollen analysis revealed that the Late Glacial is characterized by steppic taxa with prevailing Amaranthaceae, Artemisia and Poaceae. The arboreal vegetation starts to rise after 11 500 yr BP, taking a couple of millennia to be definitively attested. Holocene vegetation is characterized by the dominance of mesophilous plants. The Quercus robur type and Pinus are the most abundant taxa, followed by the Quercus cerris type, the Quercus ilex type and Ostrya–Carpinus orientalis. The first attestation of human presence can be presumed at 5000 yr BP from the contemporary presence of cereals, Juglans and Rumex. A drop in both pollen concentration and influx together with a δOcarb shift indicates increasing aridity and precedes clear and continuous human signs since 4000 yr BP. Also, a correlation between Pediastrum boryanum and fecal stanol suggests that the increase in nutrients in the water is related to human presence and pasture. An undoubted expansion of humanrelated plants occurs since 2600 yr BP when cereals, arboreal cultivated and other synanthropic non-cultivated taxa are found. A strong reduction in arboreal vegetation occurred at 2000 yr BP, when the Roman Empire impacted a landscape undergoing climate dryness in the whole Mediterranean area. In recent centuries the human impact still remains high but spots of natural vegetation are preserved. The Lake Dojran multi-proxy analysis including pollen data provides clear evidence of the importance of this approach in paleoenvironmental reconstruction. Cross-interpretation of several proxies allows us to comprehend past vegetation dynamics and human impact in the southern Balkans. A new high-resolution pollen and NPP (nonpollen palynomorph) analysis has been performed on the sediments of Lake Dojran, a transboundary lake located at the border between Greece and the former Yugoslav Republic of Macedonia (FYROM). The sequence covers the last 12 500 years and provides information on the vegetational dynamics of the Late Glacial and Holocene for the southern Balkans. Robust age model, sedimentological diatom, and biomarker analyses published previously have been the base for a multi-perspective interpretation of the new palynological data. Pollen analysis revealed that the Late Glacial is characterized by steppic taxa with prevailing Amaranthaceae, Artemisia and Poaceae. The arboreal vegetation starts to rise after 11 500 yr BP, taking a couple of millennia to be definitively attested. Holocene vegetation is characterized by the dominance of mesophilous plants. The Quercus robur type and Pinus are the most abundant taxa, followed by the Quercus cerris type, the Quercus ilex type and Ostrya–Carpinus orientalis. The first attestation of human presence can be presumed at 5000 yr BP from the contemporary presence of cereals, Juglans and Rumex. A drop in both pollen concentration and influx together with a δOcarb shift indicates increasing aridity and precedes clear and continuous human signs since 4000 yr BP. Also, a correlation between Pediastrum boryanum and fecal stanol suggests that the increase in nutrients in the water is related to human presence and pasture. An undoubted expansion of humanrelated plants occurs since 2600 yr BP when cereals, arboreal cultivated and other synanthropic non-cultivated taxa are found. A strong reduction in arboreal vegetation occurred at 2000 yr BP, when the Roman Empire impacted a landscape undergoing climate dryness in the whole Mediterranean area. In recent centuries the human impact still remains high but spots of natural vegetation are preserved. The Lake Dojran multi-proxy analysis including pollen data provides clear evidence of the importance of this approach in paleoenvironmental reconstruction. Cross-interpretation of several proxies allows us to comprehend past vegetation dynamics and human impact in the southern Balkans.


Introduction
High-resolution terrestrial archives from lakes and caves are the basis for understanding past climate changes and vegetation dynamics.Southern Europe counts a large number of extant lakes and paleo-lakes in comparison to northern African and Near Eastern regions.This is due to the geological features, geographical location and related hydrological and climatic conditions that characterize the southernmost European countries.
While a lot of Spanish, French, Italian and Greek lacustrine records have been studied since the 1970s, the vegetation dynamics of the central Balkans remained for a long time under-investigated due to a variety of natural, historical and political circumstances.Nevertheless, there are two remarkable reasons that make the area important for paleoenvironmental investigation: (1) the possibility to obtain long lacustrine records due to the high abundance of tectonic lakes, such as Dojran, Prespa, Ohrid and Maliq and (2) the A. Masi et al.: Vegetation history and paleoclimate at Lake Dojran long-lasting human occupation.Only recently has the scientific community appreciated all the potentialities of the area, which is quite rich in natural lakes, and a number of international teams are currently carrying out interdisciplinary investigations in the area.This massive boost involved either the totally new or the renewed study of several long Quaternary lacustrine sediment records: Ioannina basin (Tzedakis, 1994;Lawson et al., 2004;Roucoux et al., 2011), Lake Ohrid (Wagner et al., 2009(Wagner et al., , 2017;;Lézine et al., 2010;Sadori et al., 2016b), Lake Prespa (Panagiotopoulos et al., 2014) and Tenaghi Philippon (e.g., Tzedakis et al., 2006;Milner et al., 2012;Pross et al., 2015).
The area in general and the central-western Balkan lakes in particular (Butrint, Dojran, Ohrid, Prespa and Shkodra) are characterized by pristine nature and are important hotspots of biodiversity for aquatic and wetland life, but also for terrestrial plants and animals (Griffiths et al., 2002;Keukelaar et al., 2006;Bojovic et al., 2016).Since the publication of the paper by Willis (1994) on Balkan vegetation history, new data have become available.In particular, investigations of Holocene changes in the last 20 years concerned two lagoons from the island of Mljet (Jahns and van den Bogaard, 1998), Lake Shkodra (Zanchetta et al., 2012;Sadori et al., 2015a;Mazzini et al., 2016), Lake Butrint (Morellon et al., 2016), Lake Orestiás (Kouli, 2015;Kouli and Dermitzakis, 2010), Lake Voulkaria (Jahns, 2005), several Bulgarian mountain lakes (Tonkov and Marinova, 2005;Tonkov et al., 2008Tonkov et al., , 2016)), the peat bog Vodniza (Tonkov et al., 2018) and Lake Dojran (Athanasiadis et al., 2000).Athanasiadis et al. (2000) provided pollen results for littoral cores from Lake Dojran covering about the last 5000 years.The lacustrine record appeared to be an important archive of past environmental history and climate change of the region.The high potentiality of the archive appeared even clearer in a new longer record from the central part of the lake.This core spans the Late Glacial and the Holocene and provides a multidisciplinary dataset including geochemical (Francke et al., 2013a), diatom (Zhang et al., 2014) and biomarker (Thienemann et al., 2017) data.The new palynological study on Lake Dojran presented here represents the first detailed and continuous Holocene pollen sequence for the region based on a robust chronology and aims at (1) a high-resolution reconstruction of vegetation dynamics of Lake Dojran, (2) an evaluation of the ecosystem response to the transition from the dry-cold climate of the last glacial to the wet-hot climate of the Holocene interglacial (3), an assessment of the human impact and (4) comparison with nearby sequences in order to infer vegetation and humaninduced similarities and dissimilarities.

Site, climate and modern vegetation description
Lake Dojran (41 • 12 N, 22 • 44 E) is a transboundary lake situated at the border between the former Yugoslav Repub-lic of Macedonia (FYROM) and Greece.The lake is located at 144 m above sea level (a.s.l.) in a karst depression between two mountain chains: Belles or Belasitsa (peaking at 1870 m a.s.l.) in the northeast and Krousia or Krusa (860 m a.s.l.) in the southeast (Sotiria and Petkovski, 2004).Lake Dojran is a relict of the former Pleistocene Lake Peon, which occupied an area of about 127 km 2 (Petkovski et al., 2004).The lake has an elliptic shape with a maximum northsouth extension of 9 km and a maximum east-west extension of 7 km.With its 43 km 2 of surface, it is one of the smallest lakes in the region.Small rivers, creeks and groundwaters feed the lake.The outlet is an artificial channel, the Doiranitis River, which drains into the Axios River.The outlet is active only when the lake water level is high.It depends on rainfall, summer evaporation and in recent years on the use of water for irrigation (Griffiths et al., 2002;Sotiria and Petkovski, 2004).The result of overexploitation during the last decades of the past century was a drastic water level drop (water depth reached 4 m in 2001-2002;Sotiria and Petkovski, 2004).To restore the water depth at about 10 m as it was during the 1950s, water from nearby aquifers is pumped into the lake (Bojovic et al., 2016).
The regional climate is mainly influenced by the Mediterranean Sea, separated from the lake by the Thessaloniki Plain.The proximity to the sea and the local morphology mitigate the climate that is warmer than in other Macedonian areas (Sotiria and Petkovski, 2004;Popovska et al., 2014).For the period 1961-2000, the mean annual air temperature and precipitation were 14.3 • C and 612 mm, respectively.Precipitation occurs mainly during the mild winters, when mean temperatures of 3.7 • C are recorded.Summers are dry, with mean temperatures of 26.1 • C (Sotiria and Petkovski, 2004).
The modern vegetation of the Balkans is the result of the combined effects of climate, altitude, soil, Mediterranean Sea influence and anthropogenic activity.Mediterranean, Northern and Eastern European floral elements compose the high biodiversity of the area that is also evidenced by a high endemism (Bojovic et al., 2016).The relative proximity to the Aegean Sea (70 km) also influences the vegetation, resulting in the presence of Mediterranean taxa.The Mediterranean plant communities are characterized by evergreen forests dominated by Pinus halepensis Mill., evergreen Quercus (Quercus coccifera L., Quercus ilex L.) and Juniperus oxycedrus L., while deciduous forest includes Quercus pubescens Willd., Carpinus orientalis Mill., Ostrya carpinifolia Scop., Pistacia terebinthus L., Fraxinus ornus L. and Acer.Montane forests cover the mountain chains between 700 and 1700 m a.s.l. with Abies and Juniperus.Above 1700 m, subalpine and alpine vegetation prevails (Eastwood, 2004).

Coring, chronology and sedimentological analysis
A 717 cm long sediment sequence (Co1260) was recovered by a gravity corer and percussion piston corer in June 2011 from the Macedonian part of the lake (Fig. 1), where a hydroacoustic survey (Innomar SES-2000 compact; 10 kHz) indicated undisturbed, horizontal bedded sediments (Francke et al., 2013a).The age model is based on 13 calibrated radiocarbon ages derived from terrestrial plant material, charcoal, carbonates and bulk organic C samples (Francke et al., 2013a).The sediment sequence encompasses the last ∼ 12 500 years, i.e., the Late Glacial and the entire Holocene.Sedimentological, stable isotope (Francke et al., 2013a), diatom (Zhang et al., 2014) and biomarker (Thienemann et al., 2017) data provide a robust basis for a new study of pollen on the sequence.

Pollen analysis
Palynology has been carried out on 146 sediment samples taken from throughout core Co1260.The mean temporal resolution is 87 years.Between ca. 12 500 and 11 400 yr BP, the mean temporal resolution is 71 years, and from ca. 3250 yr BP to 2004 AD it is 66 years.For each sample, 0.29 to 0.57 g of dry sediment was chemically processed with alternating treatment of HCl (37 %), HF (40 %) and hot NaOH (10 %; Faegri and Iversen, 1989, modified) in order to remove detrital matter from the sediments.Following Stockmarr (1971), tablets containing a known amount of Lycopodium spores were added in order to estimate pollen, NPPs (non-pollen palynomorphs) and microcharcoal concentrations.Pollen concentration has been used to elaborate influx data on the basis of the sedimentation rates as inferred from the existing age model.Pollen and microcharcoal influx data are an estimation of the amount of pollen grains and charcoal fragments deposited and incorporated annually in a single unit surface (Berglund et al., 1986).The first is an estimate of the plant biomass and the second of the burnt biomass.
The pollen basis sum is constituted by terrestrial spermatophytes.For other taxa, percentages were calculated on different pollen basis sums as reported by Berglund et al. (1986).The identification of pollen morphology is based on atlases (Reille, 1992(Reille, , 1995(Reille, , 1998) ) and reference pollen collections.Pollen diagrams were drawn against both depth and timescales using the TILIA program (Grimm, 1992).All arboreal and non-arboreal pollen taxa with values higher than 2 % of the total pollen sum were used for the CONISS cluster analysis (Grimm, 1992).Pollen zones have been established following CONISS indication and visual inspection.
Pollen grains of several species belonging to genus Quercus have been divided in three morphological groups according to Smit (1973): the Quercus robur type, which includes all deciduous oaks; the Quercus cerris type, which includes all semi-evergreen oaks plus Quercus suber; and the Quercus ilex type, including all the evergreen oaks minus Quercus suber.The denomination Quercus ilex type was kept even if Quercus coccifera is the most common evergreen oak in the area.Among Poaceae, cereals have been identified and mainly include grains ascribable to the Hordeum group and Secale cereale (Andersen et al., 1979).Triticum-Avena pollen grains are present as well.Here, we present a cumulative curve for cereals.The cereal type also includes pollen grains of some wild Poaceae.Considering Asteraceae, pollen grains belonging to the two subfamilies Asteroideae and Cichorioideae have been identified.As the tribe Cichorieae, a European native, is the only one with fenestrate pollen grains (Florenzano et al., 2015), Cichorieae would be the most appropriate name instead of Cichorioideae.We keep the more generic name, as it could be more clear for the reader.Among algae, species of Pediastrum, P. simplex, P. simplex var.sturmii and P. boryanum have been recognized following Komárek and Jankovská (2001).Charcoals have been divided into three dimensional groups according to the length of the shorter axis.The particle size reveals the origin of charred fragments: the 10-50 µm group indicates regional fire, the 50-125 µm group landscape or regional fire and the > 125 µm group local fire (Whitlock et al., 2010;Sadori et al., 2015b).

Results
The pollen concentration spans from ca. 11 200 to 692 300 grains g −1 and the state of preservation of pollen is variable.The mean count of terrestrial pollen is 409 grains per sample.A total of 83 terrestrial pollen taxa are identified and include 37 trees and shrubs and 46 herb taxa.The most abundant taxa are the Quercus robur type, Pinus, the Quercus cerris type, the Quercus ilex type and Ostrya-Carpinus orientalis among arboreal plants, with Amaranthaceae, Poaceae and Artemisia among the herbs.CONISS and the results of the pollen analyses, plotted as percentage, concentration and influx values, are shown in Figs. 2 and 3.In Fig. 4, ecological groups (mesophilous, xeric, pioneer, Mediterranean, montane and synanthropic), geochemical proxies, biomarkers and planktonic diatom curves are plotted and diatom zone descriptions are summarized and simplified (for major details and a more precise description, see the paper by Zhang et al., 2014).Amaranthaceae are included in the sum of xeric taxa, even if, especially in the last millennia, some of them can be also an indication of human disturbance.Pollen zones are described also taking into consideration sedimentological and stable isotope data (Francke et al., 2013a), diatom data (Zhang et al., 2014) and biomarker analyses (Thienemann et al., 2017).
Although there are clay clasts that may have formed under subaerial conditions and thus indicate redeposition of sediments in the lowermost core section (Francke et al., 2013a), the pollen assemblage shows the herb vegetation succession typical of the Late Glacial period.AP % ranges from 31 to 45 %.Total pollen concentration (pollen grains g −1 ) varies between ca. 25 100 and 53 200, influx values between ca.2600 and 5300 and the number of taxa between 34 and 38.This phase represents the onset of Holocene reforestation.The passage is abrupt and marked by trees increasing from 17 % of D-1 to 31 % of the first sample of D-2.Herbs still prevail and arboreal vegetation is always under 45 %.Pioneer taxa (Fig. 4) play a role more important than in the previous zone, with Ephedra (> 0-4 %) slightly decreasing, while Juniperus (1-6 %), Rosaceae undiff Low TOC and the abundance of planktonic oligotrophicmesotrophic diatoms indicate low productivity.Charcoal indicates sporadic local and low regional fires.Poaceae, the Typha angustifolia type (2-4 %) and Cyperaceae (0-4 %) could have formed riparian vegetation belts along Lake Dojran shores.This vegetation could have trapped clastic matter and nutrients, explaining the limited productivity of the lake as registered by the sedimentological investigation and by the oligotrophic to mesotrophic water state as inferred from diatom analyses.AP % ranges from 36 to %.Total pollen concentration (pollen grains g Both could therefore either indicate the presence of more nutrients or shallowing water.As vegetation suggests increasing wetness, the hypothesis that changes in the lacustrine water could be due to increasing temperature favoring an increase in nutrients seems strongly supported.Mesophilous vegetation shows a continuous increasing trend that can be related to the rise in humidity suggested by δ 18 O carb at the end of the zone.Low concentration values of microcharcoals indicate the absence of local fire and sporadic regional ones.Low fire activity is also indicated by biomarkers with a low concentration of polycyclic aromatic hydrocarbon (PAH).(Francke et al., 2013a); biomarker curves (Thienemann et al., 2017) and lake features inferred by diatom data (Zhang et al., 2014) of Lake Dojran.highest value.The reduction in deciduous oaks matches a minimum in planktonic diatoms and in total diatom concentration.Pioneer taxa are on the whole reduced, with Rosaceae undiff.(1-5 %) still important and Corylus (0-4 %) increasing.Xeric taxa (Artemisia, 1-3 %; Amaranthaceae, 1-5 %; Juniperus, 0-3 %) and Asteroideae undiff.(1-4 %) are reduced.Pine is between 7 and 14 %.High values of Galium (0-5 %) and significant values of Lamiaceae (0-3 %) are still found, with a spread of Sanguisorba cf.minor (1-7 %) as well.Abies (0-1 %) shows a sporadic presence since 9800 yr BP.Microcharcoals indicate the presence of both regional and local fires.According to the pollen assemblage and sedimentological data, this time period was characterized by increasing humidity, in particular during summer.However, diatoms suggest low lake levels.AP % ranges from 76 to 93 %.Total pollen concentration (pollen grains g −1 ) varies between ca.125 800 and 342 200, influx values between ca.3700 and 13 500 and the number of taxa between 29 and 40.The Quercus robur type is dominant, with oscillation from 34 to 51 % and high influx values, in particular at 9000 yr BP.The other woodland elements (Quercus cerris type, 3-15 %; Ostrya-Carpinus orien-talis, 1-9 %; Corylus, 1-6 %), together with the first sporadic traces of Carpinus betulus (0-4 %), indicate rising temperature.Fagus is more continuously present all over the zone even if with values below 1 %.In general, the rising value of AP % is related to the increase in Abies (0-4 %), Ostrya-Carpinus orientalis and in particular of Pinus that triples its amount along this zone from 6 to 20 %.In contrast, none of the Mediterranean taxa increase.The rising of pine together with fir suggests that they both come from mountain species organized in altitudinal belts.Denser forest canopy (AP 89 % at 8400 yr BP) matches a wetness increase indicated by lower δ 18 O carb and a change in lake level suggested by diatoms (from shallow to high stands).Alisma (> 0-2 %) is attested at the very beginning of this zone.Turbid water inferred from diatoms was probably responsible for decreased Pediastrum, as a whole changing from 41 % at 8700 yr BP to 3 % at 8200 yr BP.Among the herbs, Poaceae (2-9 %) still prevail together with Sanguisorba cf.minor (1-7 %), but both of them show a decreasing trend.AP % ranges from 83 to 90 %.Total pollen concentration (pollen grains g −1 ) varies between ca.75 200 and 383 300, influx values between ca.4400 and 25 800 and the number of taxa between 28 and 44.An important vegetation change is suggested by the rapid increase in pioneer taxa, mostly Juniperus (> 0-8 %).This indicates deterioration of the forest ecosystems even if AP % is only slightly reduced.Abies (2-7 %) begins to decline.The Quercus robur type (25-39 %) and Pinus (22-35 %) are dominant and accompanied by the Quercus cerris type (4-14 %), the Quercus ilex type (0-7 %), Fagus (> 0-4 %) and Ostrya-Carpinus orientalis (1-3 %).Among arboreal plants, Olea (0-2 %), Juglans (0-1 %) and Castanea (0-1 %) appear to be more continuous than in previous zones.These three taxa are sporadically present since the early Holocene but show a clear increase since around 2500 yr BP.Olea is the first taxon to increase, followed soon after by Juglans and then by Castanea.Their contemporary and increased presence can be taken as evidence of cultivation (Mercuri et al., 2013a).This hypothesis is supported by the expansion of cereals (reaching 4 %) and the sporadic presence of Plantago since the bottom zone.The pollen assemblage suggests the use of agricultural practices.This hypothesis is confirmed by increased Glomus, suggesting soil erosion.Charcoals attesting regional fires are documented all over the zone.Diatom assemblages are evidence of shallow water and eutrophic conditions, which is confirmed by similar trends in fecal stanols and Pediastrum boryanum.At 2400 yr BP, the expansion of Pediastrum simplex var.sturmii could be an indication of increasing water temperature.

Pollen
4.9 Pollen zone D-9 (213-161 cm, 2000-1400 yr BP, duration 600 years) AP % ranges from 65 to 81 %.Total pollen concentration (pollen grains g −1 ) varies between ca.33 400 and 126 200, influx values between ca.2800 and 9600 and the number of taxa between 40 and 50.At the beginning of the zone, the arboreal vegetation rapidly drops due to the abrupt decline of Pinus (from 18 to 5 %, from 50 000 to 17 000 pollen grains g −1 ) accompanied by a slower reduction of Abies (from 7 to 1 %, from 10 000 to 1000 pollen grains g −1 ).Even though not at a similar amplitude, there are clearly similar patterns in many taxa both in concentration and influx data.Most taxa, including oaks, appear to be affected by a strong biomass decrease, even if it occurs slightly earlier than in the two mentioned conifers.The decrease started at the end of zone D-8 for the Quercus robur type (38 to 26 %, from 113 000 to 37 000 pollen grains g −1 ) and the Quercus cerris type (from 7 to 6 %, 113 000 to 37 000 pollen grains) that reach lower values ca.50 years before the drop in pine and fir.Pioneer, xeric and synanthropic taxa increase in the zone.Human presence is indicated by the high amount of synanthropic plants such as cultivated (e.g., cereals, 0-3 %; Castanea, 0-1 %; Olea; Juglans) and ruderal ones (Plantago, 0-2; Rumex, 0-2; Urtica, > 0-2).Forest clearance and the relatively high amount of xeric taxa, mainly Artemisia (1-2 %), can either be the consequence of increased aridity or human impact.The increase in Poaceae (7-15 %) and Amaranthaceae (2-5 %) could be due to low lake stands and the relative enlargement of shallow areas covered by reed beds.The productivity in the lake seems to be rather important as evidenced by TOC / TN and diatom assemblages.Charcoal assemblages show that regional and local fires are of scarce importance.
Abies remains below 2 %, suggesting that higher-altitude environments do not easily recover, being more fragile.Xeric (mainly Amaranthaceae, 0-11 %) and synanthropic taxa increase along the whole zone, in particular since 500 yr BP.
Artemisia shows rather high values in the second part of the zone.Amaranthaceae, included in the sum of xeric taxa, can also include ruderal herbs.The proximity of the lake is colonized by arboreal riparian vegetation, mainly Alnus (max 2 %).Poaceae could form a Phragmites belt around the lake like today.Shallow lake conditions are inferred from diatoms and Myriophyllum.This water plant generally grows in water up to 2 m deep (Azzella and Scarfó, 2010).Increasing temperature and eutrophic water have also been evidenced by the rising of Pediastrum taxa.Bottema et al. (1974) associated the increase in Pediastrum simplex with soil fertilization due to cattle.In the same period, biomarkers (especially the fecal stanol record) indicate strong anthropogenic impact in the catchment.Concentration and influx values of charcoal are the highest of the diagram for regional fires between 1000 and 1300 yr BP.The last part of the pollen record reflects the increasing human impact and the consequences of the recent tendencies in shaping the landscape around the lake.

General discussion and multi-proxy comparison
The Lake Dojran pollen sequence starts during the Late Glacial with the dominance of xeric taxa.Most arboreal plants are present at the bottom of the core, suggesting the presence of glacial refugia for montane and mesophilous taxa in the catchment of Lake Dojran.In particular, Pinus, the Quercus robur type and Ostrya-Carpinus orientalis peaks are recorded before 12 000 yr BP.The fluctuating presence of coniferous and deciduous taxa in glacial periods is recorded in several lakes of the central-eastern Mediterranean: Prespa (Panagiotopoulos et al., 2013), Ohrid (Sadori et al., 2016b), Ljubljana (Willis, 1994), Ioannina (Bottema et al., 1974;Tzedakis, 1994), Bulgarian mountain lakes (Tonkov and Marinova, 2005;Tonkov et al., 2008Tonkov et al., , 2016)), Monticchio (Allen et al., 2009), Vico (Magri and Sadori, 1999), Pergusa (Sadori andNarcisi, 2001), Trifoglietti (De Beaulieu et al., 2017) and Van (Litt et al., 2009).The onset of Holocene reforestation is dated at Dojran to 11 500 BP and infers increasing humidity and temperature.The data show the decline of steppe-related plants and also the presence of pioneer trees such as Rosaceae, Juniperus and Betula that constitute forest patches accompanied by Pinus and by all oak types.This behavior is typical of the succession from glacial to interglacial phases in Southern Europe (Tzedakis, 2007), as shown in Fig. 5, in which some of the available records are shown.Similarly to other lacustrine sites (Prespa, Panagiotopoulos et al., 2013;Ioannina, Lawson et al., 2004;Pergusa, Sadori and Narcisi, 2001), a couple of millennia are needed at Dojran to achieve 80 % of AP in zone D-5 at ca. 9300 yr BP, showing fairly resilient ecosystems.In particular, rising humidity is evidenced for Lake Van (Litt et al., 2009) by the increasing trend of deciduous (and semi-deciduous) oaks.In pollen zone D-2 (ca.11 500-10 900 yr BP) the AP trend shows a first start soon followed by a decrease in the percentage of mesophilous taxa that deserves consideration.It is due to a decrease in the Quercus robur type partly balanced by an increase in the Q. cerris type (peaking at 11 100 yr BP).This shift between the two oak types was probably a signal of a temporary reduction of humidity, a sort of short-term stasis of forest expansion.A similar pattern (evidenced in Fig. 5) is observed at Prespa (Panagiotopoulos et al., 2013) and Ioannina (Lawson et al., 2004) in the Balkans and at Vico (Magri and Sadori, 1999) and Pergusa (Sadori and Narcisi, 2001) in Italy.It is likely to be synchronous, and secular differences can be attributed to uncertainties in each site's age model.The pattern of zone D-2 seems to be repeated once more at Dojran in zone D-4 (10 200-9400 yr BP), where a similarity in mesophilous taxa and TOC curve trends is found (Fig. 4).This forest opening is probably less pronounced at other sites, but it matches an important change detected at higher elevations from "wetter" to "drier" taxa.At Lake Trilistnika (Tonkov et al., 2008), after a short and sharp increase in Abies soon followed by the Quercus robur type, the Quercus cerris type prevails.
The forest is definitely established at ca. 9300 yr BP (beginning of zone D-5) with AP at 80 %, increased pollen concentration and influx values and relatively increasing TOC (Figs. 3 and 4;Francke et al., 2013a).The rising AP % is paralleled by a decreasing trend in the average chain length of Arboreal pollen percentage diagram from Lake Dojran, Lake Prespa, Ioannina basin, Lake Pergusa, Lake Iznik, Lago Grande di Monticchio, Lake Vico, Orestiás and Lake Shkodra.See text for references.
vascular plant n-alkanes, also indicating increasing arboreal vegetation (Thienemann et al., 2017).Pioneer taxa, with the exception of wet-demanding Corylus that is increasing, and the light-demanding Sanguisorba cf.minor were replaced first by Abies and then at ca. 8500 yr BP by Ostrya-Carpinus orientalis and Carpinus betulus.This forest succession, resulting in an increase in "other mesophilous" and montane taxa (Fig. 4), matches the enhanced humidity (δ 18 O carb ) and high lake level inferred from diatoms.The latter is also confirmed by decreasing Poaceae, which may indicate a reduction of the lacustrine vegetation belt formed by Phragmites and/or Paspalum.The increase in humidity probably allowed for the colonization of higher altitudes and resulted in the definitive attestation of vegetation belts.Even if temperate deciduous forest prevails, mountain taxa such as Abies and Fagus probably partly replaced pioneer ones on the two mountain chains (Belles and Krousia) surrounding the lake.Arboreal vegetation (and deciduous oaks above all) prevails not only at Lake Dojran but also at other regions in the eastern and central Mediterranean, such as at Lake Prespa (Panagiotopoulos et al., 2013), in the Ioannina basin (Lawson et al., 2004), at Tenaghi Philippon (Müller et al., 2011), at Lake Iznik (Miebach et al., 2016), at Eski Acigöl (Woldring and Bottema, 2003), at Lake Van (Litt et al., 2009) and at Lake Pergusa (Sadori and Narcisi, 2001;Sadori et al., 2013).
The pollen assemblages of Lake Dojran show no clear evidence of the 8.2 ka cooling event.The zone shows rather stable vegetation conditions and a very low sedimentation rate.The event is considered the most prominent and abrupt climate change at the northern latitudes of the entire Holocene (Johnsen et al., 2001).Only some of the Mediterranean pollen records (Lake Maliq: Bordon et al., 2009;Tenaghi Philippon: Pross et al., 2009) register the 8.2 event, usually consisting of a more or less pronounced short phase with reduced precipitation (e.g., Staubwasser and Weiss, 2006;Kotthoff et al., 2008;Pross et al., 2009;Göktürk et al., 2011;Miebach et al., 2016).It is interesting to note that hydroacoustic data indicate a low lake level centered at 8.2 ka (Francke et al., 2013a) supported by a reduction of Poaceae.Water could be partially trapped in wider or longer-persisting snow cover in the mountains.The amount of Mediterranean plants is low, indicating that the water availability is related to a temperate climate and the abundance of mesophilous plants indicates wet conditions.Pross et al. (2009) suggest a thermal gradient between inland and coastal settings in the Aegean Sea with weaker winter cooling at the coast.The connection of the Dojran area with Mediterranean Sea could have mitigated the temperature reduction that, together with the resilience of vegetation, could have masked the 8.2 event impact.
Traces of synanthropic plants, including cereals, Juglans and Rumex, are found since 5000 yr BP.Biomarker data also possibly indicate the first human activities around 4500 yr BP (Thienemann et al., 2017).Athanasiadis et al. (2000) found clear signs for the first human activity at that time in two cores from the eastern edge of the lake.Co1260 (this article) was recovered from a central position in the lake and records the onset of human settlements as less pronounced, probably due to a higher distance from these settlements.However, the presence of cereals can be taken as an indication of cultivation in the area that was populated since the early Neolithic due to the migration of populations from Anato-lia and nearby Greece performing livestock farming (Kaiser and Voytek, 1983).A wide range of cereals and legumes are attested in the archaeological excavation of Anza (ca.6500-5000 yr BC) in FYROM for the Neolithic (Gimbutas, 1974).In the nearby Struma Valley (Bulgaria) the size and number of archaeological sites increase, indicating a strong increase in population, particularly in the second half of the Late Neolithic (5200-4900 yr BC; Marinova et al., 2012).Archaeological remains evidence a new increase in sedentarism during the Bronze Age, with increasing agriculture practices (Kokkinidou and Trantalidou, 1991).In Lake Dojran sediments, Juglans is attested for the first time at around 7000 yr BP, which is in agreement with a recent paper by Pollegioni et al. (2017) stating that the first exchanges of germplasm between the Near East and Aegean region are dated at the 6th millennium BP.Contemporary presence is sporadically recorded at Lake Ribno Banderishko (southwestern Bulgaria; Tonkov et al., 2002), while major quantities are found at Orestiás (Kouli, 2015).In contrast at Shkodra, Prespa and in previous data from Dojran, Juglans is attested only since 3500 yr BP in more significant quantities.The early presence of walnut in the Dojran record can be related to the economic exchange that would have spread the plant from the Near East through Turkey to Europe.The walnut tree is in fact valuable not only for the edible fruit but also for timber.
At ca. 4000 yr BP, a sharp influx and concentration drop matches an increase in Pinus, marking the start of zone D-7 and suggesting decreasing humidity.AP % remains rather stable, probably due to the expansion of Pinus.This change in forest composition probably favored the slight increase in Fagus.The other proxies available at Dojran, which are not affected by human impact, also suggest aridification and lower lake levels.Thus δ 18 O carb values are the highest of the last 9500 years, and planktonic diatoms decrease strongly.Biomarker data also indicate a drier climate around 4000 yr BP.Forest opening, occurring soon before and culminating at 4000 yr BP, is indicated in most pollen records, as shown in Fig. 5, pointing out that the phenomenon is present both in the Italian and Balkan peninsulas and is strictly related to regions under Mediterranean influence.In fact, aridification is missing both at Lake Van (Litt et al., 2009) and in the central Rila Mountains in Bulgaria where Picea shows an increasing trend since 5000 yr BP (Tonkov et al., 2016).Moreover, it is still under debate whether this change is caused by climatic change or is human induced.In central Italy, a decrease in humidity detected soon before 4000 yr BP is found in low-stand lake levels (Giraudi et al., 2011) and in speleothems (Zanchetta et al., 2016).This change in hydrology and seasonality (longer summer drought) surely affected forest plants, especially mesophilous taxa.Animal husbandry, cultivation and metallurgy were probably intensifying and accelerating the phenomenon (Sadori et al., 2004), causing forest clearance all over the central Mediterranean area (Denéfle et al., 2000;Jahns, 2005;Caroli et al., 2007;Sadori et al., 2008;Bordon et al., 2009;Di Rita and Magri, 2009;Tinner et al., 2009;Combourieu-Nebout et al., 2013;Mercuri et al., 2012Mercuri et al., , 2013a)).A major role is also to be ascribed to fire, which increased in the whole Mediterranean area (Vanniére et al., 2011;Sadori et al., 2015b).According to some authors, the use of fire and the anthropogenic degradation of forest (Marinova et al., 2012) could have favored the spread of Fagus, but a climate-driven cause should also be taken into account (Giesecke et al., 2007;Valsecchi et al., 2008).
Since 2600 yr BP, the Lake Dojran pollen assemblage shows a considerable expansion of anthropogenic indicator taxa (the synanthropic non-cultivated and cultivated plants described by Behre et al., 1990).Littoral cores studied by Athanasiadis et al. (2000) record primary and secondary anthropogenic indicators (mainly Triticum type and Secale) since ca.3500 yr BP with a lower percentage.Another major vegetation change around this date is an expansion of Juniperus that probably replaced the mesophilous forest disturbed by human activity.Also, several arboreal cultivated taxa increase in zone D-8: Castanea, Juglans and Olea.Mercuri et al. (2013a) introduced the OJC (Olea, Juglans, Castanea) sum to highlight the increasing human activity in the Mediterranean area, as these trees increase from the Bronze Age onwards all over Italy.Mercuri et al. (2013b) made a step forward by adding seven selected anthropogenic nonarboreal pollen indicators (APIs) common in archaeological sites to investigate the shaping of the cultural landscape (Marignani et al., 2017).
At Lake Dojran, cereal percentages are low but quite significant (around 5 %) and attest a well-developed agricultural system (Sadori et al., 2016a).This is due to self-pollination and the consequent under-representation in pollen diagrams (van Zeist et al., 1975;Faegri and Iversen, 1989).Pollen related to grazing or disturbed areas like Plantago lanceolata and Rumex are very scarce.Pasture land was probably reduced, while agriculture seems to have played an important role in the economy.Marinova et al. (2012) pointed out that after a period with no archaeological evidence, a remarkable socioeconomical expansion is attested since the 6th century BC at around 2500 yr BP.
Either a climate change or a large-scale human impact can be invoked to explain the strong reduction of Pinus and Abies that occurred during D-9 after 2000 yr BP.First Greeks, followed by Romans and Byzantines could have regionally cut them for the valuable timber.Macedonia was indeed an important region for timber harvesting since the 5th century AD and probably earlier.Fir and pine were the standard ship timber, and naval power depended on them (Harris, 2013).Their pronounced decrease is again related to the presence of Juniperus that typically takes advantage in more degraded environments.At the same time, both the concentration and influx reach very low values, mirroring decreasing PAHs.Cereal, Castanea, Juglans and Olea cultivation together with ruderal and weed taxa attest a strong human impact.His-torical reconstructions confirm that in Macedonia the culmination of the growth in both cereals and walnut occurred during the mid-Roman and Late Antiquity periods (1650-1500 yr BP; Izdebski et al., 2015).
A strong reduction of AP % (Fig. 5), caused by a decrease in oaks or pines, is found at many sites around 2000 BP.In particular, a Pinus drop is found at Prespa at 2300 yr BP (Panagiotopoulos et al., 2013), at Trilistnika at 2000 yr BP (Tonkov et al., 2008) and probably also at Eski Acigöl (Woldring and Bottema, 2003) even if for the Turkish site the chronology remains uncertain.In all sites this change marks forest reduction.It can once more be interpreted either as a transition from wetter to drier conditions or as a strong human-induced forest clearance.Climatic data are available from tree-ring-based reconstructions of Central Europe (Büntgen et al., 2011).The authors rule out a decrease in total reconstructed precipitation and temperature anomalies at 2100 yr BP.A global climate change seems, however, to be ruled out for this period (Bond et al., 1997).
The new spread of Pinus occurring in D-10 might not indicate a recovery of the forest as mesophilous tree percentages are decreasing.Pines can in fact have a pioneer role in anthropogenic-influenced landscapes (Litt et al., 2012), as they quickly grow in degraded areas such as those colonized by junipers.At ca. 700 yr BP, high pollen concentration and influx values match high TOC, increased polycyclic aromatic hydrocarbon and fecal stanol concentrations and the slightly increased Cichorioideae that could be related to pastoralism (Florenzano et al., 2015).Although decreased temperatures are detected in geochemical proxies, the Little Ice Age (LIA) is not evident in pollen data.The LIA could be seen in the forest recovery indicated by increasing influx values (Fig. 3) but not in the pollen percentage assemblage that still remains high.Strong human activities consisting of livestock farming, fire use and cultivation may mask the climate signal and overlap the natural changes of the LIA.Strong land use is still present in the top samples, with hints of Olea, Juglans and Vitis cultivation in the last few centuries.The expansion of these taxa corresponds to the economic and demographic one reported by Gogou et al. (2016) at around 900 yr BP as a consequence of a new political order, when the Macedonian region became of central importance for the Byzantine Empire.Olea and Vitis cultivation has been and still is of primary importance in the Byzantine diet (Xoplaki et al., 2016).Grapes are currently the primary product of the Dojran area, even if today there are few farms growing Vitis vinifera on altogether 263 ha (Bojovic et al., 2016).The increase in Poaceae recorded in D-10 can be ascribed to the presence of a littoral belt.Modern vegetation suggests the presence of either Paspalum distichum (water finger grass) or Phragmites australis (common reed).Common reeds have been recently used in the area for different purposes, primarily to produce special traps for fishing (Bojovic et al., 2016).

Conclusions
The new high-resolution pollen record from Lake Dojran draws the vegetation history of the area between FYROM and Greece for the last 12 500 years, evidencing the regional response to climate forcing.The pollen record covers the environmental changes from the Late Glacial until today passing from a natural undisturbed landscape to one in which increasing anthropogenic influences overlap climate change.These new data, together with sedimentological, biomarker and diatom data available from the same core, highly contribute to a better understanding of the environmental history, including both climatic and human evidence.
A steppe characterized by Amaranthaceae, Artemisia and Poaceae prevails during the Late Glacial period.The beginning of the Holocene reforestation at Lake Dojran is dated at 11 500 yr BP, which is similar to other Mediterranean records.It consists of a false start of AP also found in other sites, like Orestiás, Lake Prespa, Ioannina and Lake Pergusa, and preceding the real expansion of the Holocene forest.Two millennia were necessary to reach maturity in the forests dominated by deciduous Quercus.Mesophilous vegetation dominated for the entire Holocene, but a welldeveloped forest is found until ca.4000 yr BP.The first human traces are recorded around 5000 yr BP with the presence of cereals, Juglans and Rumex.Synanthropic taxa rise considerably during the Bronze Age and particularly in the Roman age.Around 2000 yr BP, the arboreal pollen and mainly Pinus show a strong reduction that is detectable at most sites of the Mediterranean basin.Even if the attestation of drier conditions can be advocated to explain the abrupt change in vegetation, the forest clearance made by the Roman Empire would have been strong enough to affect vegetation on a regional scale.Present day vegetation is human influenced, but the natural vegetation is still preserved, confirming the importance of the region as a biodiversity conservation area.
The comparison of the Dojran pollen record with the regional ones evidences similarities in the reafforestation dynamics at the beginning of the Holocene.The detailed sequence clarifies and better describes some peculiarities like the characteristic interruption in afforestation at the beginning of the Holocene (between ca. 10 800 and 11 500 yr BP).Although this behavior was present in other proxies, the Dojran sequence clearly evidences this dynamic, allowing for the identification of the same behavior in the region.The high arboreal cover that characterized the mid-Holocene is more evident at Dojran where the fluctuations in the arboreal pollen percentage are minimal despite the high analytical detail.Finally, the clear AP decrease related to the strong reduction of pine since 2200 yr BP is now detectable in the regional proxies to which it has been linked.The presented data represent the first Late Glacial and Holocene continuous sequence of Lake Dojran.The high-resolution pollen analysis and the available multi-proxy dataset provide a unique contribution to the reconstruction of vegetation dynamics and their relationship with paleoenvironmental changes and the human impact of the entire region.

Figure 1 .
Figure 1.(a) Location of Lake Dojran and the other sites mentioned in the paper.(b) Detail of the lake and its surrounding, the hydrological basin and the location of the analyzed core Co1260.The map is taken from Basemap Esri (https://www.esri.com/en-us/home,last access: 8 March 2018).

Figure 3 .
Figure 3. Concentration (a) and influx (b) pollen diagrams of selected taxa and charcoals.